Fig. 3. miR-675-3' reduces levels of MMP1 and MMP13. A, C; RT-qPCR for MMP1 and MMP13 of RNA from human articular chondrocytes treated with negative control mimic or mimic for miR-675-3' measured three days post transfection. B, D, determination of enzymatic activity for MMP1 (B) and MMP13 (D) in cell culture supernatants of cells treated with negative control or miR-675-3' mimic after three days of transfection. E, western blot for MMP1 form cell culture supernatants on day three after treatment with negative control inhibitor or inhibitor against miR-675-3'. Statistical analysis was carried out with a paired, two-tailed t-test. N, negative control mimic/inhibitor; 675-3', mimic/inhibitor for miR-675-3'. Statistical significance was set at p ≤ 0.05. TRFE, transferrin.